Post by flounder9 on Aug 24, 2012 13:09:38 GMT -5
Diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting disease within white-tailed deer (Odocoileus virginianus) herds in North America
Effects of age, sex, polymorphism at PRNP codon 96, and disease progression
Bruce V. Thomsen1
David A. Schneider
Katherine I. O’Rourke
Thomas Gidlewski
James McLane
Robert W. Allen
Alex A. McIsaac
Gordon B. Mitchell
Delwyn P. Keane
Terry R. Spraker
Aru Balachandran
U.S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA (Thomsen)
U.S. Department of Agriculture, Agricultural Research Service, Pullman, WA (Schneider, O’Rourke)
U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, Fort Collins, CO (Gidlewski)
Canadian Food Inspection Agency, Battleford, Saskatchewan, Canada (McLane)
Canadian Food Inspection Agency, Prince Albert, Saskatchewan, Canada (Allen)
Canadian Food Inspection Agency, Saskatoon, Saskatchewan, Canada (McIsaac)
National and OIE Reference Laboratory for Scrapie and CWD, Canadian Food Inspection Agency, Ottawa Laboratory–Fallowfield, Ottawa, Ontario, Canada (Mitchell, Balachandran)
University of Wisconsin, Wisconsin Veterinary Diagnostic Laboratory, Madison, WI (Keane)
Colorado State University Diagnostic Laboratory, Fort Collins, CO (Spraker)
↵1 Bruce V. Thomsen, National Veterinary Services Laboratories, 1920 Dayton Avenue, Ames, IA 50010. bruce.v.thomsen@aphis.usda.gov
Abstract
An effective live animal diagnostic test is needed to assist in the control of chronic wasting disease (CWD), which has spread through captive and wild herds of white-tailed deer (Odocoileus virginianus) in Canada and the United States. In the present study, the diagnostic accuracy of rectal mucosa biopsy sample testing was determined in white-tailed deer from 4 CWD-infected captive herds. Specifically, the current study compared the immunohistochemical detection of disease-associated prion protein in postmortem rectal mucosa biopsy samples to the CWD status of each deer as determined by immunodiagnostic evaluations of the brainstem at the obex, the medial retropharyngeal lymph node, and the palatine tonsil. The effects of age, sex, genotype, and disease progression were also evaluated. Diagnostic sensitivity on rectal biopsy samples for CWD in white-tailed deer ranged from 63% to 100%; the pooled estimate of sensitivity was 68% with 95% confidence limits (95% CLs) of 49% and 82%. However, diagnostic sensitivity was dependent on genotype at prion protein gene (PRNP) codon 96 and on disease progression as assessed by obex grade. Diagnostic sensitivity was 76% (95% CLs: 49%, 91%) for 96GG deer but only 42% (95% CLs: 13%, 79%) for 96GS deer. Furthermore, diagnostic sensitivity was only 36% for deer in the earliest stage of disease (obex grade 0) but was 100% for deer in the last 2 stages of preclinical disease (obex grades 3 and 4). The overall diagnostic specificity was 99.8%. Selective use of antemortem rectal biopsy sample testing would provide valuable information during disease investigations of CWD-suspect deer herds.
vdi.sagepub.com/content/24/5/878.abstract?etoc
Friday, August 24, 2012
Diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting disease within white-tailed deer (Odocoileus virginianus) herds in North America
chronic-wasting-disease.blogspot.com/2012/08/diagnostic-accuracy-of-rectal-mucosa.html
TSS
Effects of age, sex, polymorphism at PRNP codon 96, and disease progression
Bruce V. Thomsen1
David A. Schneider
Katherine I. O’Rourke
Thomas Gidlewski
James McLane
Robert W. Allen
Alex A. McIsaac
Gordon B. Mitchell
Delwyn P. Keane
Terry R. Spraker
Aru Balachandran
U.S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA (Thomsen)
U.S. Department of Agriculture, Agricultural Research Service, Pullman, WA (Schneider, O’Rourke)
U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, Fort Collins, CO (Gidlewski)
Canadian Food Inspection Agency, Battleford, Saskatchewan, Canada (McLane)
Canadian Food Inspection Agency, Prince Albert, Saskatchewan, Canada (Allen)
Canadian Food Inspection Agency, Saskatoon, Saskatchewan, Canada (McIsaac)
National and OIE Reference Laboratory for Scrapie and CWD, Canadian Food Inspection Agency, Ottawa Laboratory–Fallowfield, Ottawa, Ontario, Canada (Mitchell, Balachandran)
University of Wisconsin, Wisconsin Veterinary Diagnostic Laboratory, Madison, WI (Keane)
Colorado State University Diagnostic Laboratory, Fort Collins, CO (Spraker)
↵1 Bruce V. Thomsen, National Veterinary Services Laboratories, 1920 Dayton Avenue, Ames, IA 50010. bruce.v.thomsen@aphis.usda.gov
Abstract
An effective live animal diagnostic test is needed to assist in the control of chronic wasting disease (CWD), which has spread through captive and wild herds of white-tailed deer (Odocoileus virginianus) in Canada and the United States. In the present study, the diagnostic accuracy of rectal mucosa biopsy sample testing was determined in white-tailed deer from 4 CWD-infected captive herds. Specifically, the current study compared the immunohistochemical detection of disease-associated prion protein in postmortem rectal mucosa biopsy samples to the CWD status of each deer as determined by immunodiagnostic evaluations of the brainstem at the obex, the medial retropharyngeal lymph node, and the palatine tonsil. The effects of age, sex, genotype, and disease progression were also evaluated. Diagnostic sensitivity on rectal biopsy samples for CWD in white-tailed deer ranged from 63% to 100%; the pooled estimate of sensitivity was 68% with 95% confidence limits (95% CLs) of 49% and 82%. However, diagnostic sensitivity was dependent on genotype at prion protein gene (PRNP) codon 96 and on disease progression as assessed by obex grade. Diagnostic sensitivity was 76% (95% CLs: 49%, 91%) for 96GG deer but only 42% (95% CLs: 13%, 79%) for 96GS deer. Furthermore, diagnostic sensitivity was only 36% for deer in the earliest stage of disease (obex grade 0) but was 100% for deer in the last 2 stages of preclinical disease (obex grades 3 and 4). The overall diagnostic specificity was 99.8%. Selective use of antemortem rectal biopsy sample testing would provide valuable information during disease investigations of CWD-suspect deer herds.
vdi.sagepub.com/content/24/5/878.abstract?etoc
Friday, August 24, 2012
Diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting disease within white-tailed deer (Odocoileus virginianus) herds in North America
chronic-wasting-disease.blogspot.com/2012/08/diagnostic-accuracy-of-rectal-mucosa.html
TSS
